Journal: Journal of Translational Medicine
Article Title: Evaluation of the anti-angiogenic properties of the new selective α V β 3 integrin antagonist RGDechiHCit
doi: 10.1186/1479-5876-9-7
Figure Lengend Snippet: Representative immunohistochemical sections (5 μm) of subcutaneously injected Matrigel plugs . ECs were identified (light blue arrowheads) by lectin staining, which gave a brown reaction product, as described in Methods. Both c(RGDf[NMe]V) and RGDechiHCit treatment reduced the number of invading cells from the edge (black arrows) to the core of implanted Matrigel plug. Analysis was conducted in 20 randomly chosen cross-sections per each group. Bar: 400 nm. * = p < 0.05 vs VEGF. Error bars show SEM.
Article Snippet: ECs were identified by lectin immunohistochemical staining (Sigma-Aldrich) [ ] and quantitative analysis was performed using digitized representative high resolution photographic images, with a dedicated software (Image Pro Plus; Media Cybernetics, Bethesda, MD, USA) as previously described [ ].
Techniques: Immunohistochemical staining, Injection, Staining